Technical Note: RNA Extraction from FFPE Samples
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چکیده
Existing methods rely primarily on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids such as RNA. In contrast, the catalytic CAT5TM technology developed by Cell Data Sciences and included in the RNAstormTM kit greatly accelerates the removal of formaldehyde damage and allows the use of milder conditions, resulting in markedly improved recovery of amplifiable RNA. This greatly enhances the chances of success in recovering high yields of good quality nucleic acids suitable for a variety of applications, including RNA-seq or other next-generation sequencing (NGS), qPCR, microarray, and gene expression analysis.
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